The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

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Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification (Volume 326) (Methods in Enzymology, Volume 326)

edited by Jeremy Thorner, Scott D. Emr, John N. Abelson

Jeremy W Thorner; Scott D Emr; John N Abelson

Simon, Melvin I.

Morgan Kaufmann Publishers

Brooks/Cole

Methods in enzymology -- v. 326, < 327, 328 >, Methods in enzymology -- v. 326, 327, 328., San Diego, Calif, London, California, 2000

Methods in enzymology, v. 326, 327, 328, etc, San Diego ; London, ©2000

United States, United States of America

Elsevier Ltd., San Diego, Calif, 2000

October 2000

lg1105291

{"edition":"1","isbns":["0121822273","9780121822279"],"publisher":"Academic Press","series":"Methods in Enzymology 326"}

Includes bibliographical references and indexes.
LIBRARY HAS: Pt. A, B, C.

Content:
Contributors to volume 326
Pages ix-xi
Preface
Pages xiii-xiv
Jeremy Thorner, Scott D. Emr, John N. Abelson
Volumes in series
Pages xv-xxxiii
[1] The all purpose gene fusion Original Research Article
Pages 3-7
Jon Beckwith
[2] A practical guide to the construction and use of lac fusions in Escherichia coli Original Research Article
Pages 11-35
Nicholas J. Hand, Thomas J. Silhavy
[3] Tagging exported proteins using Escherichia coli alkaline phosphatase gene fusions Original Research Article
Pages 35-47
Colin Manoil
[4] Applications of gene fusions to green fluorescent protein and flow cytometry to the study of bacterial gene expression in host cells Original Research Article
Pages 47-73
Raphael H. Valdivia, Lalita Ramakrishnan
[5] IVET and RIVET: Use of gene fusions to identify bacterial virulence factors specifically induced in host tissues Original Research Article
Pages 73-96
James M. Slauch, Andrew Camilli
[6] Identification of exported bacterial proteins via gene fusions to Yersinia pseudotuberculosis invasin Original Research Article
Pages 97-104
Micah J. Worley, Fred Heffron
[7] Use of imidazoleglycerolphosphate dehydratase (His3) as a biological reporter in yeast Original Research Article
Pages 107-119
Joe Horecka, George F. Sprague Jr.
[8] Use of fusions to human thymidine kinase as reporters of gene expression and protein stability in Saccharomyces cerevisiae Original Research Article
Pages 120-133
Sofie R. Salama
[9] Use of fusions to thymidine kinase Original Research Article
Pages 134-146
Christiaan Karreman
[10] In Vivo analysis of lacZ fusion genes in transgenic Drosophila melanogaster Original Research Article
Pages 146-159
Stephen Small
[11] Utility of the secreted placental alkaline phosphatase reporter enzyme Original Research Article
Pages 159-164
Bryan R. Cullen
[12] Fusions to imidazopyrazinone-type luciferases and aequorin as reporters Original Research Article
Pages 165-174
Satoshi Inouye
[13] Novel methods for chemiluminescent detection of reporter enzymes Original Research Article
Pages 175-202
Corinne E.M. Olesen, Yu-Xin Yan, Betty Liu, Dina Martin, Brian D'Eon, Ray Judware, Chris Martin, John C. Voyta, Irena Bronstein
[14] Fusions to chloramphenicol acetyltransferase as a reporter Original Research Article
Pages 202-221
Clayton Bullock, Cornelia Gorman
[15] Fusions to ОІ-lactamase as a reporter for gene expression in live mammalian cells Original Research Article
Pages 221-241
Gregor Zlokarnik
[16] Purification of proteins using polyhistidine affinity tags Original Research Article
Pages 245-254
Joshua A. Bornhorst, Joseph J. Falke
[17] Generating fusions to glutathione S -transferase for protein studies Original Research Article
Pages 254-270
Donald B. Smith
[18] Use of the Strep - tag and streptavidin for detection and purification of recombinant proteins Original Research Article
Pages 271-304
Arne Skerra, Thomas G.M. Schmidt
[19] Streptavidin-containing chimeric proteins: design and production Original Research Article
Pages 305-311
Takeshi Sano, Charles R. Cantor
[20] Fusions to maltose-binding protein: Control of folding and solubility in protein purification Original Research Article
Pages 312-321
Deepali Sachdev, John M. Chirgwin
[21] Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli Original Research Article
Pages 322-340
Edward R. LaVallie, Zhijian Lu, Elizabeth A. Diblasio-Smith, Lisa A. Collins-Racie, John M. McCoy
[22] Affinity purification of recombinant proteins fused to calmodulin or to calmodulin-binding peptides Original Research Article
Pages 340-362
Peter Vaillancourt, Chao-Feng Zheng, Danny Q. Hoang, Lisa Breister
[23] The SВ·tag fusion system for protein purification Original Research Article
Pages 362-376
Ronald T. Raines, Mark McCormick, Thomas R. Van Oosbree, Robert C. Mierendorf
[24] Fusions to self-splicing inteins for protein purification Original Research Article
Pages 376-418
Ming-Qun Xu, Henry Paulus, Shaorong Chong
[25] Fusion proteins containing cellulose-binding domains Original Research Article
Pages 418-429
Jae-Seon Park, Hae-Sun Shin, Roy H. Doi
[26] Biotinylation of proteins in vivo and in vitro using small peptide tags Original Research Article
Pages 430-440
Millard G. Cull, Peter J. Schatz
[27] Biotinylation of proteins in vivo: A useful posttranslational modification for protein analysis Original Research Article
Pages 440-458
John E. Cronan Jr., Kelynne E. Reed
[28] Methods for generating multivalent and bispecific antibody fragments Original Research Article
Pages 461-479
Ian Tomlinson, Philipp Holliger
[29] Design and use of phage display libraries for the selection of antibodies and enzymes Original Research Article
Pages 480-505
Francesca Viti, Fredrik Nilsson, Salvatore Demartis, Adrian Huber, Dario Neri
[30] Use of an Lpp-OmpA fusion vehicle for bacterial surface display Original Research Article
Pages 506-516
Charles F. Earhart
[31] Identification of bacterial class I accessible proteins by disseminated insertion of class I epitopes Original Research Article
Pages 516-527
Dolph Ellefson, Adrianus W.M. van der Velden, David Parker, Fred Heffron
[32] Bordetella pertussis adenylate cyclase toxin: A vehicle to deliver CD8-positive T-cell epitopes into antigen-presenting cells Original Research Article
Pages 527-542
Pierre Guermonprez, Catherine Fayolle, Gouzel Karimova, Agnes Ullmann, Claude Leclerc, Daniel Ladant
[33] Use of anthrax toxin fusions to stimulate immune responses Original Research Article
Pages 542-551
Christopher C. Zarozinski, R. John Collier, Michael N. Starnbach
[34] Use of fusions to viral coat proteins as antigenic carriers for vaccine development Original Research Article
Pages 551-569
Peter G. Stockley, Robert A. Mastico
Author index
Pages 571-600
Subject index
Pages 601-617

2013-12-20