Human monoclonal antibodies have potential applications in a broad spectrum of biomedical areas.

duxiu/initial_release/40108739_.zip

zlibzh/no-category/JOHN J.LANGONE HELEN VAN VUNAKIS, Nathan P. Colowick, Nathan P. Kaplan, John J Langone, Helen Van Vunakis/METHODS IN ENZYMOLOGY VOLUME 92 IMMUNOCHEMICAL TECHNIQUES PART E MONOCLONAL ANTIBOIES AND GENERAL IMMUNOASSAY METHODS_30799594.pdf

Methods in Enzymology, Volume 92: Immunochemical Techniques, Part E: Monoclonal Antibodies and General Immunoassay Methods

Helen Van Vunakis; John J Langone; Giovanni Di Sabato; Johannes Everse

Langone, John J.; Van Vunakis, Helen

Academic Press, Incorporated

Morgan Kaufmann Publishers

Brooks/Cole

Methods in enzymology, v. 70, 73, 74, 84, 92, 93, 108, 116, 121, 132, 150, 162, 163, New York, 1980-<1988>

Methods in enzymology, vol.(92), New York, 1983

Methods in enzymology, San Diego, c1980-

United States, United States of America

March 28, 1983

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topic: Antibodies, Monoclonal; Immunoassay; Immunochemistry; Immunodosage; Immunochimie; Immunoglobulines monoclonales

Type: 英文图书

Bookmarks:
1. (p1) Section 1. Hybridoma Technology
1.1. (p3) A. Production of Monoclonal Antibodies with Selected Applications
1.1.1. (p3) 1. Human-Human Monoclonal Antibody-Producing Hybridomas: Technical Aspects
1.1.2. (p17) 2. Methods of Enhancing the Frequency of Antigen-Specific Hybridomas
1.1.3. (p26) 3. Spleen Cell Analysis and Optimal Immunization for High-Frequency Production of Specific Hybridomas
1.1.4. (p36) 4. Estimation of the Number of Monoclonal Hybridomas in a Cell-Fusion Experiment
1.1.5. (p39) 5. Use of Human Endothelial Culture Supernatant (HECS) as a Growth Factor for Hybridomas
1.1.6. (p47) 6. Production, Purification, and Characterization of Antigen-Specific Murine Monoclonal Antibodies of IgE Class
1.1.7. (p66) 7. Production and Properties of Monoclonal Antibodies to Guinea Pig Ia Antigens
1.1.8. (p86) 8. Purification of Murine MHC Antigens by Monoclonal Antibody Affinity Chromatography
1.1.9. (p110) 9. Monoclonal Antibodies against HLA Products and Their Use in Immunoaffmity Production
1.1.10. (p138) 10. Immunoassay for Sequence-Specific DNA-Protein Interactions
1.2. (p147) B. Detection and Assessment of Monoclonal Antibodies
2. (p255) Section 2. Immunoassay of Antigens and Antibodies
2.1. (p257) A. Labeling of Antigens and Antibodies
2.1.1. (p257) 21. Methods for the Attachment of Haptcns and Proteins to Erythrocytes
2.1.2. (p277) 22. Iodine Monochloride (ICI) Iodination Techniques
2.1.3. (p292) 23. Application of High-Performance Liquid Chromatography to Characterize Radiolabeled Peptides for Radioimmunoassay, Biosynthesis, and Microsequence Studies of Polypeptide Hormones
2.1.4. (p309) 24. Preparation of Stable Radioiodinated Polypeptide Hormones and Proteins Using Polyacrylamide Gel Electrophoresis
2.2. (p336) B. Separation Methods in Immunoassay
2.2.1. (p336) 25. Noncentrifugati0n Immunoassays: Novel Systems
2.2.2. (p345) 26. Use of Activated Thiol-Sepharose in a Separation Method for Enzyme Immunoassay
2.2.3. (p359) 27. Affinity Exclusion: A New Method for the Separation of Free and Bound Fractions in Enzyme Immunoassay
2.2.4. (p366) 28. Use of Chromatography Tubes in the Separatioa of Bound and Free Fractions in Radioimmunoassay
2.3. (p377) C. Immunoassay Methods
2.3.1. (p377) 29. Enzyme-Linked Immunoelectrotransfer Blot Techniques (EITB) for Studying the Specificities of Antigens and Antibodies Separated by Gel Electrophoresis
2.3.2. (p391) 30. Quantitative, Single-Tube, Kinetic-Dependent Enzyme-Linked Immunosorbent Assay (k-ELISA)
2.3.3. (p403) 31. Immunoassay Using Antigen-Coated Plastic Tubes and Radiolabeled or Enzyme-Labeled Protein A
2.3.4. (p413) 32. Colorimetric Immunoassays Using Flavin Adenine Dinucleotide as Label
2.3.5. (p426) 33. Bioluminescent Immunoassays
2.3.6. (p432) 34. Immunoassay by Electrochemical Techniques
2.3.7. (p445) 35. Metalloimmunoassay: Principles and Practice
2.3.8. (p458) 36. Fluorescence Fluctuation Immunoassay
2.3.9. (p472) 37. Methods and Application of Hapten-Sandwich Labeling
2.3.10. (p489) 38. Use of Lectin-Antibody Conjugates for Quantitation and Titration of Antigens and Antibodies
2.3.11. (p498) 39. Partition Affinity Ligand Assay (PALA) for Quantifying Haptens, Macromolecules, and Whole Cells
2.3.12. (p523) 40. Preparation and Applications of Multivalent Antibodies with Dual Specificity
2.4. (p543) D. Data Analysis
2.4.1. (p543) 41. LIGAND: A Computerized Analysis of Ligand Binding Data
2.4.2. (p577) 42. Two Simple Programs for the Analysis of Data from Enzyme-Linked Immunosorbent Assays (ELISA) on a Programmable Desk-Top Calculator
2.4.3. (p589) 43. Determination of Affinity and Specificity of Anti-Hapten Antibodies by Competitive Radioimmunoassay
2.4.4. (p601) 44. Methods of Measuring Confidence Limits in Radioimmunoassay

theme: Antibodies, Monoclonal; Immunoassay; Immunochemistry; Immunodosage; Immunochimie; Immunoglobulines monoclonales

FROM THE PREFACE: Several papers in this volume deal with advances in hybridoma technology and complement the chapter by Galfri and Milstein that appeared in Volume 73 of this series. Methods to enhance the efficiency of producing desired fusion products, to simplify the basic procedures involved, and to assess the specificity and other properties of monoclonal antibodies are discussed. Representative examples illustrate how a battery of monoclonal antibodies with different specificities can be used to study various immunochemical and biochemical problems. Other chapters cover additional procedures that can be used to label antigens and antibodies, separate antigen-antibody complexes, and process data. Some papers classified under one heading or contained in another volume of the series include methods that can be applied in other specific areas. For example, cytotoxicity tests described in Volume 93 may be used to screen supernatant fluids in hybridoma experiments if cytotoxic activity is a desirable property of the monoclonal antibody

2024-06-13