Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development. In Polyamines: Methods and Protocols , leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study. The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.

lgrsnf/507.pdf

Pegg, Anthony E. (edt); Casero, Robert A., Jr. (edt)

edited by Anthony E. Pegg, Robert A. Casero, Jr

Anthony E. Pegg, Robert Anthony Casero

United States, United States of America

Includes bibliographical references and index.

Current status of the polyamine research field -- Anthony E. Pegg and Robert A. Casere, Jr.
Exploring polyamine biosynthetic diversity through comparative and functional genomics -- Anthony J. Michael
Characterization of genes for polyamine modulon -- Kazuei Igarashi and Keiko Kashiwagi
Posttranscriptional regulation of gene expression in epithelial cells by polyamines -- Lan Xiao and Jian-Ying Wang
Identification, chemical synthesis, and biological functions of unusual polyamines produced by extreme thermophiles -- Tairo Oshima, Toshiyuki Moriya, and Yusuke Terui
Polyamine block of inwardly rectifying potassium channels -- Harley T. Kurata, Wayland W.L. Cheng, and Colin G. Nichols
Carcinogenesis studies in mice with genetically engineered alterations in polyamine metabolism -- David J. Feith
Transgenic rodents with altered SSAT expression as models of pancreatitis and altered glucose with lipid metabolism -- Anne Uimari ... [et al.]
Use of (gyro) gy and spermine synthase transgenic mice to study functions of spermine -- Xiaojing Wang and Anthony E. Pegg
Simple assay for mammalian spermine oxidase : a polyamine catabolic enzyme implicated in drug response and disease -- Andrew C. Goodwin, Tracy R. Murray-Stewart, and Robert A. Casero, Jr.
Characterization, assay, and substrate specificity of plant polyamine oxidases -- Panagiotis N. Moschou and Kalliopi A. Roubelakis-Angelakis
Assay of deoxyhypusine synthase activity -- Jong Hwan Park, Edith C. Wolff, and Myung Hee Park
Identification and assay of allosteric regulators of S-adenosylmethionine decarboxylase -- Erin K. Willert, Lisa N. Kinch, and Margaret A. Phillips
Protocols for studying antizyme expression and function -- Noriyuki Murai, Yasuko Murakami, and Senya Matsufuji
Identification, assay and functional analysis of the antizyme inhibitor family -- Chaim Kahana
Posttranscriptional regulation of ornithine decarboxylase -- Shannon L. Nowotarski, Sofia Origanti, and Lisa M. Shantz
Identification and assays of polyamine transport systems in Escherichia coli and Saccharomyces cerevisiae -- Keiko Kashiwagi and Kazuei Igarashi
Genetic and biochemical analysis of protozoal polyamine transporters -- Marie-Pierre Hasne and Buddy Ullman
Heparan sulfate proteoglycan-mediated polyamine uptake -- Johanna Welch ... [et al.]
Polyamine transport sysems in mammalian cells and tissues -- Takeshi Uemura and Eugene W. Gerner
Procedures to evaluate the importance of dietary polyamines -- Paul Acheampong, Mary J. Macleod, and Heather M. Wallace
Determination of N1,N12-diacetylspermine in urine : a novel tumor marker -- Masao Kawakita ... [et al.]
Spermidine/spermine-N1-acetyltransferase in kidney ischemia reperfusion injury -- Kamyar Zahedi and Manoocher Soleimani
Use of polyamine metabolites as markers for stroke and renal failure -- Kazuei Igarashi and Keiko Kashiwagi
Methods to evaluate alterations in polyamine metabolism caused by Helicobacter pylori infection -- Alain P. Gobert, Rupesh Chaturvedi, and Keith T. Wilson
High-resolution capillary gas chromatography in combination with mass spectrometry for quantification of three major polyamines in postmortem brain cortex -- Gary Gang Chen ... [et al.]
Spermine synthase deficiency resulting in X-linked intellectual disabiltiy (Snyder-Robinson Syndrome) -- Charles E. Schwartz, Xaiojing Wang, Roger E. Stevenson, and Anthony E. Pegg
Methylated polyamines as research tools -- Alex R. Khomutov ... [et al.]
Fluorescent substrates for polyamine catabolic enzymes and transport -- Koichi Takao and Akira Shirahata
Use of polyamine derivatives as selective histone deacetylase inhibitors -- Patick M. Woster
Measurement of polyamine pKa values -- Ian S. Blagbrough, Abdelkader A. Metwally, and Andrew J. Geall
Polyamine analysis by LC-MS -- Merja R. Hakkinen.

Polyamines
Preface
Contents
Contributors
Part I: Introduction
Part II: Identification of Polyamine Metabolic Enzymesand Characterization of Polyamine-Regulated Genesand Ion Channels
Part III: Transgenic Models for Study of Polyamine Function
Part IV: Enzymes Involved in Polyamine Catabolismand Posttranslational Protein Modification
Part V: Regulation of Polyamine Content
Part VI: Polyamine Transport and Uptake
Part VII: Polyamines and Disease
Part VIII: Chemistry and Analysis
Index

2024-04-19