The critically acclaimed laboratory standard, **Methods in Enzymology**, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences

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Immunochemical Techniques, Part B, Volume 73: Volume 73: Immunochemical Techniqies Part B (Methods in Enzymology)

Nathan P. Colowick, John J. Langone, Helen Van Vunakis, Nathan P. Kaplan

Helen Van Vunakis; John J Langone; Giovanni Di Sabato; Johannes Everse

Langone, John J.; Van Vunakis, Helen

Academic Press, Incorporated

Morgan Kaufmann Publishers

Brooks/Cole

United States, United States of America

August 28, 1981

lg1105419

{"edition":"1","isbns":["0121819736","9780121819736"],"publisher":"Academic Press","series":"Methods in Enzymology 73"}

topic: Immunochemistry; Immunoassay; Immunochemistry

Type: 英文图书

Bookmarks:
1. (p1) Section 1. Production of Antibodies
1.1. (p3) 1. Preparation of Monoclonal Antibodies: Strategies and Procedures
1.2. (p46) 2. Production of Antisera with Small Doses of Imnumogen: Multiple Intradermal Injections
1.3. (p52) 3. Production of Specific Antisera by Immunization with Precipitin Lines
1.4. (p57) 4. Polymers for the Sustained Release of molecules: Their Use in a Sinsle-Step Method of Immunization
2. (p78) Section 2. Labeling
2.1. (p79) 5. ~(125)Iodinated Tracers for Hapten-Specific Radioimmunoassays
2.2. (p112) 6. Radioiodinationn by Use of the Bolton-humter and Related Reagents
2.3. (p127) 7. Use of ~(125)I-Labeled Anti-2,4-Dinitrophenyl(DNP) Antibodies as a Tracer in Solid-Phase Radioimmunoassays
2.4. (p138) 8. Tritiation of Proteins to High Specific Activity: Application to Radioimmunoassay
2.5. (p147) 9. Methods for the Preparation of Enzyme-Antibody Conjugates for Use in Enzyme Immunoassay
2.6. (p166) 10. Purification of Peroxidase-Conjugated Antibody for Enzyme Immunoassay by Affinity Chromatography on Concanavalin A
2.7. (p176) 11. Preparation of Protein A-Enzyme Monoconjugate and Its Use as a Reagent in Enzyme Immunoassays
2.8. (p191) 12. Effect of Catalytic Hydrogenation of Cellular Lipid and Fatty Acid on the Susceptibility of Tumor Cells to Humoral Immune Killing
3. (p202) Section 3. Separation Techniques in Immunoassay
3.1. (p203) 13. Use of Particulate Immunosorbents in Radioimmunoassay
3.2. (p224) 14. Antibody-Coated Plastic Tubes in Radioimmunoassay
3.3. (p239) 15. Quantitation of Antibodies Immobilized on Plastics
3.4. (p245) 16. Radioimmtmoassay of Peptide Hormones Using Killed Staphylococcus aureus as a Separating Agent
3.5. (p254) 17. Microencapsulation of Antibody for Use in Radioimmunoassay
3.6. (p261) 18. High-Pressure Liquid Chromatography and Radioimmunoassay for the SpeCific and Quantitative Determination of Endorphins and Related Peptides
3.7. (p275) 19. Qualitative and Quantitative Analysesof Arachidonic Acid Metabolites by Combined High-Performance Liquid Chromatography and Radioimmunoassay
4. (p289) Section 4. Immunoassay Methods for the Detection of Antigens and Antibodies
4.1. (p289) A. Immunoassay in Gels
4.1.1. (p291) 20. Single Radial Immunodiffusion
4.1.2. (p306) 21. The Two-Cross Immunodiffusion Technique Determining Diffusion Coefficients and Precipitating Titers of Antigen and Antibody
4.1.3. (p319) 22. Antigen Quantitation by a Reverse Hemolytic Assay
4.1.4. (p326) 23. The Reverse plaque-Forming Cell Assay
4.1.5. (p339) 24. Electroimmunoasssay
4.1.6. (p370) 25. Quantitation of Antigens by Densitometric Scanning of Immunoelectrophoretic Precipitates
4.2. (p381) B. Immunoassays Dependent on Radiolabeled Tracers
4.2.1. (p383) 26. Ultrasensitive Enzymic Radioimmunoassay
4.2.2. (p394) 27. Autoradiographic Immunoassay (ARIA)
4.2.3. (p406) 28. Methods for Binding Cells to Plastic: Application to Solid Phase Immunoassays for Cell-Surface Antigens
4.2.4. (p418) 29. Microprocedures for Quantitative Immunochemical Analysis of Antigenic Molecules and Antigenic Determinants
4.2.5. (p436) 30. The Use of [~(125)I]C1q Subcomponent for the Measurement of Complement-Binding Antibodies on Cell Surfaces
4.2.6. (p442) 31. Use of Protein A-Bearing Staphylococci for the Immunoprecipitation and Isolation of Antigens from Cells
4.2.7. (p459) 32. Recovery of Antigen from Staphylococcal Protein A-Antibody Adsorbents
4.3. (p471) C. Immunoassays Dependent on Enzyme-Labeled Tracers
4.3.1. (p471) 33. Masnetic Solid-Phase Enzyme Immunoassay for the Quantitation of Antigens and Antibodies: Application to Human Immunoglobulin E
4.3.2. (p482) 34. The Amplified ELISA: Principles of and Applications for the Comparative Quantitation of and Subclass Antibodies and the Distribution of Antibodies and Antigens in Biochemical Separates
4.3.3. (p523) 35. Steric Hindrance Enzyme Immunoassay (SHEIA) Using β-Galactosidase as an Enzyme Label and Maleimide Derivative of Hapten (or Antigen) for Enzyme Coupling
4.3.4. (p543) 36. Antibody Quantitation Using the Unlabeled AntibodyEnzyme Immunoassay (UNLIM) Method
4.3.5. (p550) 37. Use of Antibodies and the Primary Enzyme lmmunoassay (PEIA) to Study Enzymes: The Arylsulfatase A-Anti-Arylsulfatase A System
4.4. (p578) D. Immunoassay of Immunoglobulins
4.4.1. (p578) 38. Immunonephelometric Assay for Immunoglobulins Released by Cultured Lymphocytes
4.4.2. (p597) 39. Solid-Phase Radioimmunoassay for Immunoglobulins and Influenza Antibodies
4.4.3. (p608) 40. A Luminol-Assisted Competitive Binding Immunoassay of Human Immunoglobulin G
4.4.4. (p616) 41. Preparation and Radioimmunoassay of IgM Domains
4.4.5. (p625) 42. Quantitation of Secretory Protein Levels by Radioimmunoassay
4.4.6. (p634) 43. Double-Antibody Radioimmunoassay for IgE
4.4.7. (p646) 44. Radioimmunoassay for IgE Using Paper Disks
4.4.8. (p656) 45. Enzyme-Linked Immunosorbent Assays (ELISA) for Immunoglobulin E and Blocking Antibodies
4.4.9. (p666) 46. Development and Clinical Application of Radioimmunoassay Techniques for Measuring Low Levels of Immunoglobulin Classes G, A, M, D, and E in Cerebrospinal and Other Body Fluids

theme: Immunochemistry; Immunoassay; Immunochemistry

Content:
Contributors to volume 73
Pages ix-xi
Preface
Page xiii
John J. Langone, Helen Van Vunakis
Volumes in series
Pages xv,xvii-xxii
[1] Preparation of monoclonal antibodies: Strategies and procedures Original Research Article
Pages 3-46
G. GalfrГЁ, C. Milstein
[2] Production of antisera with small doses of immunogen: multiple intradermal injections Original Research Article
Pages 46-52
Judith L. Vaitukaitis
[31] Production of specific antisera by immunization with precipitin lines Original Research Article
Pages 52-57
Jens KrГёll
[4] Polymers for the sustained release of macromolecules: Their use in a single-step method of immunization Original Research Article
Pages 57-75
Robert Langer
[5] 125 Iodinated tracers for hapten-specific radioimmunoassays Original Research Article
Pages 79-112
John E.T. Corrie, William M. Hunter
[6] Radioiodination by use of the Bolton-Hunter and related reagents Original Research Article
Pages 112-127
John J. Langone
[7] Use of 125 I-labeled anti-2,4-dinitrophenyl (DNP) antibodies as a general tracer in solid-phase radioimmunoassays Original Research Article
Pages 127-138
A.R. Neurath
[8] Tritiation of proteins to high specific activity: Application to radioimmunoassay Original Research Article
Pages 138-147
Brian F. Tack, Ronald L. Wilder
[9] Methods for the preparation of enzyme-antibody conjugates for use in enzyme immunoassay Original Research Article
Pages 147-166
M.J. O'Sullivan, V. Marks
[10] Purification of peroxidase-conjugated antibody for enzyme immunoassay by affinity chromatography on concanavalin A Original Research Article
Pages 166-175
J. Arends
[11] Preparation of protein A—Enzyme monoconjugate and its use as a reagent in enzyme immunoassays Original Research Article
Pages 176-191
Avadhesha Surolia, Debkumar Pain
[12] Effect of catalytic hydrogenation of cellular lipid and fatty acid on the susceptibility of tumor cells to humoral immune killing Original Research Article
Pages 191-199
Seymour I. Schlager
[13] Use of particulate immunosorbents in radioimmunoassay Original Research Article
Pages 203-224
Leif Wide
[14] Antibody-coated plastic tubes in radioimmunoassay Original Research Article
Pages 224-239
George H. Parsons Jr.
[15] Quantitation of antibodies immobilized on plastics Original Research Article
Pages 239-244
John E. Herrmann
[16] Radioimmunoassay of peptide hormones using killed Staphylococcus aureus as a separating agent Original Research Article
Pages 245-253
Shag-Yao Yin
[17] Microencapsulation of antibody for use in radioimmunoassay Original Research Article
Pages 254-261
Franklin Lim, Robert J. Buehler
[18] High-pressure liquid chromatography and radioimmunoassay for the specific and quantitative determination of endorphins and related peptides Original Research Article
Pages 261-275
J.G. Loeber, J. Verhoef
[19] Qualitative and quantitative analyses of arachidonic acid metabolites by combined high-performance liquid chromatography and radioimmunoassay Original Research Article
Pages 275-288
Iftekhar Alam, Lawrence Levine
[20] Single radial immunodiffusion Original Research Article
Pages 291-305
Jean-Pierre Vaerman
[21] The two-cross immunodiffusion technique for determining diffusion coefficients and precipitating titers of antigen and antibody Original Research Article
Pages 306-319
Biserka Pokrić, Zvonimir Pučar
[22] Antigen quantitation by a reverse hemolytic assay Original Research Article
Pages 319-326
Giuseppe A. Molinaro, William C. Eby, Christine A. Molinaro
[23] The reverse plaque-forming cell assay Original Research Article
Pages 326-338
Giuseppe A. Molinaro, William C. Eby, Christine A. Molinaro
[24] Electroimmunoassay Original Research Article
Pages 339-369
Carl-Bertil Laurell, E. John McKay
[25] Quantitation of antigens by densitometric scanning of immunoelectrophoretic precipitates Original Research Article
Pages 370-383
Jens KrГёll
[26] Ultrasensitive enzymic radioimmunoasy Original Research Article
Pages 383-394
Ih-Chang Hsu, Robert H. Yolken, Curtis C. Harris
[27] Autoradiographic immunoassay (ARIA) Original Research Article
Pages 394-406
Elmar W. Weiler, Meinhart H. Zenk
[28] Methods for binding cells to plastic: Application to solid phase immunoassays for cell-surface antigens Original Research Article
Pages 406-418
C.H. Heusser, T.W. Stocker, R.H. Gisler
[29] Microprocedures for quantitative immunochemical analysis of antigenic molecules and antigenic determinants Original Research Article
Pages 418-436
Kazimierz Malinowski, Wladyslaw Manski
[30] Use of [ 125 I]C1q subcomponent for the measurement of complement-binding antibodies on cell surfaces Original Research Article
Pages 436-442
Philip S. Shepherd, Christopher J. Dean
[31] Use of protein A—bearing staphylococci for the immunoprecipitation and isolation of antigens from cells Original Research Article
Pages 442-459
Steven W. Kessler
[32] Recovery of antigen from staphylococcal protein A—Antibody adsorbents Original Research Article
Pages 459-471
J.M. MacSween, S.L. Eastwood
[33] Magnetic solid-phase enzyme immunoassay for the quantitation of antigens and antibodies: Application to human immunoglobulin E Original Research Article
Pages 471-482
Jean-Luc Guesdon, Stratis Avrameas
[34] The amplified ELISA: Principles of and applications for the comparative quantitation of class and subclass antibodies and the distribution of antibodies and antigens in biochemical separates Original Research Article
Pages 482-523
J.E. Butler
[35] Steric hindrance enzyme immunoassay (SHEIA) using ОІ-Galactosidase as an enzyme label and maleimide derivative of hapten (or antigen) for enzyme coupling Original Research Article
Pages 523-542
Albert Castro, Nobuo Monji
[36] Antibody quantitation using the unlabeled antibody enzyme immunoassay (UNLIM) method Original Research Article
Pages 543-550
David E. Lenz
[37] Use of antibodies and the primary enzyme immunoassay (PEIA) to study enzymes: The arylsulfatase A-anti-arylsulfatase A system Original Research Article
Pages 550-578
Edward Aneuwelt
[38] Immunonephelometric assay for immunoglobulins released by cultured lymphocytes Original Research Article
Pages 578-596
Jose MuГ±oz, Gabriel Virella, H. Hugh Fudenberg
[39] Solid-phase radioimmunoassay for immunoglobulins and influenza antibodies Original Research Article
Pages 597-608
Harry Daugharty
[40] A luminol-assisted competitive binding immunoassay of human immunoglobulin G Original Research Article
Pages 608-615
Leroy S. Hersh, William P. Vann, Sally A. Wilhelm
[41] Preparation and radioimmunoassay of IgM domains Original Research Article
Pages 616-625
Akira Shimizu, Shinichiro Watanabe
[42] Quantitation of secretory protein levels by radioimmunoassay Original Research Article
Pages 625-634
J.L. Klein, J.R. Dawson
[43] Double-antibody radioimmunoassay for IgE Original Research Article
Pages 634-645
S.L. Dunnette, G.J. Gleich
[44] Radioimmunoassay of IgE using paper disks Original Research Article
Pages 646-656
Miroslav Ceska
[45] Enzyme-linked immunosorbent assays (ELISA) for immunoglobulin E and blocking antibodies Original Research Article
Pages 656-666
Donald R. Hoffman
[46] Development and clinical application of radioimmunoassay techniques for measuring low levels of immunoglobulin classes G, A, M, D, and E in cerebrospinal and other body fluids Original Research Article
Pages 666-691
S.T. Nerenberg, R. Prasad
Author index
Pages 693-716
Subject index
Pages 717-739

Contents: Pt. A. [without Special Title]--pt. B. [without Special Title] -- Pt. C. [without Special Title] -- Pt. D. Selected Immunoassays / Edited By John J. Langone, Helen Van Vunakis -- Pt. E. Monoclonal Antibodies And General Immunoassays Methods / Edited By John J. Langone, Helen Van Vunakis -- Pt. F. Conventional Antibodies, Fc Receptors, And Cytoxicity / Edited By John J. Langone, Helen Van Vunakis. -- Pt. G. Separation And Characterization Of Lymphoid Cells / Edited By Giovanni Di Sabato, John J. Langone -- Pt. I. Hybridoma Technolgy And Monoclonal Antibodies / Edited By John J. Langone, Helen Van Vunakis. Edited By Helen Van Vunakis, John J. Langone. Editors Vary. Includes Bibliographical References And Indexes.

2013-12-20