In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course. Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy

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nexusstc/Enzymes. Biochemistry, Biotechnology, Clinical Chemistry/8c5611ccaf7df0edbd7d1180ea25b082.pdf

zlib/Biology and other natural sciences/Plants: Agriculture and Forestry/T. Palmer and P.L. Bonner (Auth.)/Enzymes. Biochemistry, Biotechnology, Clinical Chemistry_2338234.pdf

Trevor Palmer, Philip L.Bonner,Woodhead Publishing

Trevor Palmer; Philip L.R. Bonner

Trevor Palmer; Philip L. R Bonner

Philip Bonner, Trevor Palmer

Palmer, T, Bonner, P L

Horwood Publishing Ltd

United Kingdom and Ireland, United Kingdom

Elsevier Ltd., Oxford, 2011

2nd ed, Chichester, 2007

2, FR, 2007

lg1169712

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Content:
Front matter , Pages i-iii
Copyright , Page iv
Authors’ Preface , Pages xiv-xv
1 - An Introduction to Enzymes , Pages 2-13
2 - The Structure of Proteins , Pages 14-43
3 - The Biosynthesis and Properties of Proteins , Pages 44-66
4 - Specificity of Enzyme Action , Pages 67-75
5 - Monomeric and Oligomeric Enzymes , Pages 76-83
6 - An Introduction to Bioenergetics, Catalysis and Kinetics , Pages 85-104
7 - Kinetics of Single-Substrate Enzyme-Catalysed Reactions , Pages 105-125
8 - Enzyme Inhibition , Pages 126-152
9 - Kinetics of Multi-Substrate Enzyme-Catalysed Reactions , Pages 153-172
10 - The Investigation of Active Site Structure , Pages 173-188
11 - The chemical nature of enzyme catalysis , Pages 189-221
12 - The Binding of Ligands to Proteins , Pages 222-238
13 - Sigmoidal Kinetics and Allosteric Enzymes , Pages 239-254
14 - The Significance of Sigmoidal Behaviour , Pages 255-272
15 - Investigation of Enzymes in Biological Preparations , Pages 274-292
16 - Extraction and Purification of Enzymes , Pages 293-314
17 - Enzymes as Analytical Reagents , Pages 315-327
18 - Instrumental Techniques Available for Use in Enzymatic Analysis , Pages 328-342
19 - Applications of Enzymatic Analysis in Medicine, Forensic Science and Industry , Pages 343-355
20 - Biotechnological Applications of Enzymes , Pages 356-376
21 - Genomics, Proteomics and Bioinformatics , Pages 377-396
Answers to Problems , Pages 397-402
Abbreviations , Pages 403-404
Index , Pages 405-416

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.<br><br><ul><li>Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject</li><li>Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification</li><li>Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy</li></ul>

In recent years, there have been considerable developments in techniques for the investigation and utilization of enzymes. With the assistance of a co-author, this popular student textbook has now been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

2014-05-06