The study of gene expression in neural tissue brings to bear some of the newest and most productive scientific technology to one of the most exciting and fastest growing areas. This volume describes methods for the study of the expression of specific genes frequently found in very low abundance. The key features and benefits of this volume include: convenient benchtop format; methods presented for easy adaptation to new systems; comprehensive protocols included for in situ hybridization, comparison of in situ and immuno-chemical methods for the study of regulatory molecules; gene transfer; the study of the regulation of gene expression; the enhancer trap method; quantifying rare mRNAs; isolation and use of cell lines; the homology approach for receptor cloning; trouble shooting. Specific protocols included for the assessment of the expression of the following genes: calbindin, corticotrop in-releasing hormone, cytokines and neurotrophic factors, dystropin, FMRF amide, glucose transporter, insulin-like growth factor interphotoreceptor retinoid binding protein, muscarinic acetylcholine receptor, neuropeptide Y, neurotrophin receptor, nerve growth factor and its receptor, and proopiomelanocort in (POMC), and weaver

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zlib/Biology and other natural sciences/P Michael Conn;Paul A Hargrave;Errol B De Souza;John N Fain;Toshio Narahashi;All authors/Methods in neurosciences. 23_2462953.pdf

Providing Pharmacological Access to the Brain: Alternate Approaches. Edited by Thomas R. Flanagan, Dwaine F. Emerich and Shelley R. Winn

Gene Expression In Neural Tissues, Volume 9: Volume 9: Gene Expression In Neural Tissues (methods In Neurosciences)

Methods in Neurosciences: Gene Expression in Neural Tissues (Methods in Neurosciences)

Paul A. Hargrave; Errol B. De Souza; John N. Fain; Toshio Narahashi; Jon E. Levine; Thomas R. Flanagan; Dwaine F. Emerich; Shelley R. Winn; E. Ronald De Kloet; Win Sutanto; A. Ian Smith; M. Ian Phillips; Dwight Evans; Stuart C. Sealfon; Gobinda Sarkar; Patrick C. Roche; Jacob Kraicer; S. Jeffrey Dixon; Michael L. Johnson; Johannes D. Veldhuis; J. Regino Perez-Polo

Divisions of Neuroscience and Reproductive Services Oregon Regional Primate Research Center Department of Physiology and Pharmacology P Michael Conn, Ph.D.; P. Michael Conn

edited by P. Michael Conn. Vol.9, Gene expression in neural tissues

Series Editor-p. Michael Conn

Morgan Kaufmann Publishers

Brooks/Cole

Methods in neurosciences -- v. 9, Methods in neurosciences -- v. 9, San Diego, Calif, California, 1992

United States, United States of America

San Diego, London, England, 1992

Spiral edition, October 1992

June 1992

lg1289068

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Includes bibliographical references and index

Calbindin D28k Gene Expression In Neurodegenerative Diseases / Anthony M. Iacopino, William B. Rhoten And Sylvia Christakos -- Corticotropin-releasing Factor Gene Expression / Toshihiro Suda -- Gene Expression Of Cytokines And Neurotrophic Factors In Human Brain Tumors / Terry Lichtor And Mark E. Gurney -- Dystrophin Gene Expression In Neuronal Tissues / Jamel Chelly And Axel Kahn -- Neuropeptide Fmrfamide Gene Expression / Julian F. Burke [and Others] -- Rat Brain/hep G2 Glucose Transporter Gene Expression In Brain / Mohan K. Raizada [and Others] -- Insulin-like Growth Factor Ii Gene Expression / Thomas J. Lauterio -- Expression Of Mouse Interphotoreceptor Retinoid-binding Protein Gene During Development / Gregory I. Liou [and Others] -- Analysis Of Muscarinic Acetylcholine Receptor Expression And Function / Neil M. Nathanson [and Others] -- Expression Of Human Neuropeptide Y Gene In Neuronal Cell Lines / Carolyn A. Minth -- Neuropeptide Y Gene Expression / Brian J. Morris --^ Neurotrophin Receptor Gene Expression / Christopher J. Earley And Gerald A. Higgins -- Detection Of Nerve Growth Factor And One Of Its Receptors / Diane F. Hill [and Others] -- Assessment Of Proopiomelanocortin Gene Expression In Brain / Phyllis M. Wise [and Others] -- Weaver Gene Expression In Central Nervous System / Lazaros C. Triarhou -- In Situ Hybridization And Immunohistochemical Methods In Study Of Regulatory Molecules / Martin Schalling -- Using Mrna In Situ Hybridization To Localize Wnt-3 And Wnt-3a Expression In Developing Neural Tube / Henk Roelink And Roel Nusse -- In Situ Hybridization For Neurofilament And Gap-43 Mrna In Primary Sensory Neurons In Conjunction With Nerve Growth Factor Receptor Radioautography / W. Tetzlaff [and Others] -- Gene Regulation Analysis By Lipopolyamine-mediated Dna Transfer In Primary Neurons / F. Barthel [and Others] -- Retrovirus-mediated Gene Transfer Into Mouse Cerebellar Cultures / Masaaki Tsuda --^ Analysis Of Cis-acting Elements Of Oxytocin Gene By Dna-mediated Gene Transfer / Stephane Richard And Hans H. Zingg -- Identification Of Cis-regulatory Sequences Required For Induction Of Neurotensin/neuromedin N Gene Expression In Pc12 Cells / Edward Kislauskis And Paul R. Dobner -- Characterization Of Neuron-specific Transcription Factors In Drosophila Melanogaster / Wayne A. Johnson -- Dna-protein Interactions In Phenotypically Identified Neurons In Discrete Regions Of Intact Brain / Caroline L. Szymeczek-seay And David E. Millhorn -- Enhancer Trap Method In Drosophila / Marek Mlodzik And Yasushi Hiromi -- Use Of Northern Blotting For Quantifying Rare Mrnas In Neurons Of Developing Peripheral Nervous System / Sean Wyatt And Alun M. Davies -- Dopamine D2 Receptors / Richard D. Todd, Janice M. Hickok And Karen L. O'malley -- Cloning Of Dopamine Receptors / James R. Bunzow, Qun-yong Zhou And Olivier Civelli --^ Cloning Of Human Thyrotropin-releasing Hormone Cdna Using Degenerate Oligonucleotide Probes And Tetramethylammonium Chloride / Marie A. Tavianini, Peter J. Gkonos And Bernard A. Roos. (cont) In Vivo Analysis Of Gene Expression In Central Catecholamine Cells / V. Leviel And Nicole Faucon-biguet. Edited By P. Michael Conn. Includes Bibliographical References And Index.

Gene expression facilitates the use of the newest and most productive scientific technology. This volume describes methods for the study of the expression of specific genes frequently found in low abundance.

2014-11-04