MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression.
This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process – or stops it – is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; MicroRNA expression, maturation and functional analysis; and MicroRNAs and disease.

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nexusstc/Identification of Viral MicroRNAs/e398cbf560464eeacc5c4e13d319d86f.pdf

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MicroRNA Methods, Volume 427 (Methods in Enzymology) (Methods in Enzymology)

MicroRNA Methods (ISSN Book 427)

Micro: RNA Methods

edited by John J. Rossi, Gregory J. Hannon

Christopher S. Sullivan; Adam Grundhoff

Sidney P. Colowick; Nathan Oram Kaplan

Gregory J Hannon; John J Rossi

Rossi, John J.

Academic Press, Incorporated

Morgan Kaufmann Publishers

Elsevier Academic Press

Brooks/Cole

Methods in enzymology -- v. 427., San Diego, Calif, California, 2007

Methods in enzymology, vol. 427, Amsterdam, cop. 2007

Elsevier Ltd., San Diego, California, 2007

United States, United States of America

1 edition, September 4, 2007

lg1105343

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Referenced by: doi:10.1261/rna.2183803 doi:10.1101/gr.2845604 doi:10.1038/ng1794 doi:10.1128/jvi.00831-06 doi:10.1073/pnas.0408192102 doi:10.1371/journal.ppat.0020023 doi:10.1128/jvi.79.20.13094-13104.2005 doi:10.1128/jvi.00200-06 doi:10.1038/ng1793 doi:10.1111/j.1462-5822.2005.00598.x doi:10.1101/gad.862301 doi:10.1128/jvi.79.18.12095-12099.2005 doi:10.1261/rna.2326106 doi:10.1038/nature04836 doi:10.1007/bf00818163 doi:10.1099/vir.0.80449-0 doi:10.1186/1742-4690-1-44 doi:10.1038/sj.onc.1209915 doi:10.1126/science.1096781 doi:10.1038/nmeth746 doi:10.2217/17460794.1.2.233 doi:10.1128/jvi.79.14.9301-9305.2005 doi:10.1016/j.molcel.2005.09.012 doi:10.1038/nature03576 doi:10.1038/sj.emboj.7600491

Includes bibliographical references and indexes.

Content:
Identification of Viral MicroRNAs Review Article
Pages 1,3-23
Christopher S. Sullivan, Adam Grundhoff
Robust Machine Learning Algorithms Predict MicroRNA Genes and Targets Review Article
Pages 25-49
Pål Sætrom, Ola Snøve Jr.
Identification of Virally Encoded MicroRNAs Review Article
Pages 51-63
SГ©bastien Pfeffer
Computational Methods for MicroRNA Target Prediction Review Article
Pages 65-86
Yuka Watanabe, Masaru Tomita, Akio Kanai
In Vitro and In Vivo Assays for the Activity of Drosha Complex Review Article
Pages 87,89-106
Yoontae Lee, V. Narry Kim
Microarray Analysis of miRNA Gene Expression Review Article
Pages 107-122
J. Michael Thomson, Joel S. Parker, Scott M. Hammond
Cloning and Detecting Signature MicroRNAs from Mammalian Cells Review Article
Pages 123-138
Guihua Sun, Haitang Li, John J. Rossi
Approaches for Studying MicroRNA and Small Interfering RNA Methylation In Vitro and In Vivo Review Article
Pages 139-154
Zhiyong Yang, Giedrius Vilkaitis, Bin Yu, Saulius KlimaЕЎauskas, Xuemei Chen
Analysis of Small RNA Profiles During Development Review Article
Pages 155-169
Toshiaki Watanabe, Yasushi Totoki, Hiroyuki Sasaki, Naojiro Minami, Hiroshi Imai
Dissecting MicroRNA‐Mediated Gene Regulation and Function in T‐Cell Development Review Article
Pages 171-189
Tin Ky Mao, Chang‐Zheng Chen
Investigation of MicroRNA Alterations in Leukemias and Lymphomas Review Article
Pages 191,193-213
George Adrian Calin, Carlo Maria Croce
Discovery of Pathogen‐Regulated Small RNAs in Plants Review Article
Pages 215-227
Surekha Katiyar‐Agarwal, Hailing Jin
Protocols for Expression and Functional Analysis of Viral MicroRNAs Review Article
Pages 229-243
Eva Gottwein, Bryan R. Cullen
Contributors to Volume 427
Pages XI-XIII
Contents
Pages V-IX
Author Index
Pages 245-256
Contents of Previous Volumes
Pages XV-XL
Subject Index
Pages 257-261
Series Editors
Page II

2013-12-20