In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim­ special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech­ and for the publication of extended abstracts.

lgrsnf/R:\062020\springer2\10.1007%2F978-1-4615-7133-9.pdf

nexusstc/Handbook of Biological Confocal Microscopy/fd26e791ec31c8c99d78e663dc90c357.pdf

scihub/10.1007/978-1-4615-7133-9.pdf

zlib/Medicine/Shinya Inoué (auth.), James B. Pawley (eds.)/Handbook of Biological Confocal Microscopy_5925966.pdf

Springer London, Limited

United States, United States of America

Springer Nature, New York, NY, 2012

sm23210993

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Front Matter....Pages i-xiii
Foundations of Confocal Scanned Imaging in Light Microscopy....Pages 1-14
Fundamental Limits in Confocal Microscopy....Pages 15-26
Quantitative Fluorescence Imaging with Laser Scanning Confocal Microscopy....Pages 27-39
The Pixelated Image....Pages 41-51
Laser Sources for Confocal Microscopy....Pages 53-67
Non-laser Illumination for Confocal Microscopy....Pages 69-76
Objective Lenses For Confocal Microscopy....Pages 77-86
Size and Shape of The Confocal Spot: Control and Relation to 3D Imaging and Image Processing....Pages 87-91
The Intermediate Optical System of Laser-scanning Confocal Microscopes....Pages 93-103
Intermediate Optics in Nipkow Disk Microscopes....Pages 105-111
The Role of the Pinhole in Confocal Imaging Systems....Pages 113-126
Photon Detectors for Confocal Microscopy....Pages 127-139
Manipulation, Display, and Analysis of Three-Dimensional Biological Images....Pages 141-150
Three-dimensional Imaging on Confocal and Wide-field Microscopes....Pages 151-161
Direct Recording of Stereoscopic Pairs Obtained From Disk-scanning Confocal Light Microscopes....Pages 163-168
Fluorophores for Confocal Microscopy: Photophysics and Photochemistry....Pages 169-178
Image Contrast in Confocal Light Microscopy....Pages 179-195
Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy....Pages 197-205
A Comparison of Various Optical Sectioning Methods: The Scanning Slit Confocal Microscope....Pages 207-214
Back Matter....Pages 215-232

In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J.P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the limƯ special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical techƯ and for the publication of extended abstracts

2020-08-30