Whole-cell biocatalysis is a promising and emerging field of biological science that is mostly based on advancements in biosystems engineering for the production of useful products such as chemicals, fuels, and pharmaceuticals. It is a unique platform that provides an efficient catalytic system at affordable cost and with low maintenance. Recent studies have proven that whole-cell catalysis is very useful in conducting many complex and complicated chemical reactions that could be executed with greater ease and in an eco-friendly manner in ambient conditions with zero/minimal ecological effect. This has made whole-cell biocatalysis very popular and a center of significance in the field of modern biological catalysis. This new volume offers a comprehensive understanding of whole-cell biocatalysis, beginning with an introduction and an overview of relevant tools and techniques. It presents the basic as well as the advanced concepts of whole-cell biocatalysis, thus acting as a complete guide for the reader. It looks at the promising developments that have taken place in the past decade and discusses the application of computational tools, the use of metabolic flux systems, and the role of metabolic networks for the cell system development for whole-cell catalysis. The chapters explore whole-cell biocatalysis for the production of chemicals, of fuels, and for environmental restoration, with case studies and success stories that showcase new developments in the technology. This volume provides a valuable presentation of whole-cell biocatalysis for graduate students, faculty, industry professionals, and others working in biochemistry and bio-systems engineering. A comprehensive book on whole-cell biocatalysis covering basic as well as advanced concepts. Latest developments including computational tools, use of metabolic flux systems, and the role of metabolic networks for the cell system development are discussed using case studies to demonstrate practical uses of whole-c

lgli/Pamidimarri S. Whole-Cell Biocatalysis. Next-Generation Technology for Green Synthesis of Pharmaceutical, Chemicals, and Biofuels_2024.pdf

lgrsnf/Pamidimarri S. Whole-Cell Biocatalysis. Next-Generation Technology for Green Synthesis of Pharmaceutical, Chemicals, and Biofuels_2024.pdf

zlib/Chemistry/Biochemistry/Sudheer D. V N. Pamidimarri, Sushma Chauhan, Balasubramanian Velramar/Whole-Cell Biocatalysis_29474624.pdf

Pamidimarri S.D.V.N., Chauhan S., Velramar B. (ed.)

Canada - English Language, Canada

1, PS, 2024

2023

{"isbns":["1774914425","9781774914427"],"last_page":666,"publisher":"Apple Academic Press","source":"libgen_rs"}

Cover
Half Title
Whole-Cell Biocatalysis: Next-Generation Technology for Green Synthesis of Pharmaceutical, Chemicals, and Biofuels
Copyright
About the Editors
Contents
Contributors
Abbreviations
Acknowledgment
Preface
Part I: Introduction to Whole-Cell Catalysis
1. Whole‐Cell Biocatalysts: Introduction, Origin, and Concept
Abstract
1.1 Introduction
1.2 Catalysis vs. Biocatalysis
1.2.1 Traditional Catalysis
1.2.2 Concept of Biocatalysis
1.2.3 Origin of WCB
1.3 Concept of Whole-Cell Biocatalysis
1.4 Prerequisites for Optimal Chassis Selection
1.5 Strategies Used for Development of WCB
1.5.1 Blocking of Competitive Pathways
1.5.2 Expression of Heterologous Pathways or Enzyme
1.5.3 Single and Multistep Biocatalysis
1.5.4 Enhancing the Precursor Supply and Co-Factor Regeneration
1.6 Tools for Engineering the WCBS
1.6.1 DNA Elements and Their Use for Controlled Expression
1.6.2 Genome Editing Using Molecular Tools
1.6.3 Computational Tools for In-Silico Analysis
1.7 Conclusions and Future Prospective
Keywords
References
2. Tools and Techniques for the Development of Whole‐Cell Biocatalysis
Abstract
2.1 Introduction
2.2 System Design and Approach for Building a WCB
2.2.1 Genome Scale Models (GSMS)-Computational Simulator of Cell Metabolism
2.3 Engineering Inherent Enzymes for Target WCB Process
2.3.1 Directed Evolution
2.3.2 Rational Design for Selective Engineering
2.3.3 Semi-Rational Design
2.3.4 De Novo Design
2.4 Tools for Expression of Enzymes/Enzyme Cascade
2.4.1 Plasmid and Copy Number
2.4.2 Promoters
2.4.3 Codon Bias
2.5 Tools for Gene Editing
2.5.1 Zinc Finger Nuclease System
2.5.2 Transcription Activator-Like Effectors (TALEs)
2.5.3 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)
2.5.3.1 Mechanism
2.5.3.2 For Fabrication of Cells
2.5.4 Chromosomal Integration
2.6 Tools for Gene Regulation
2.6.1 Regulatory RNAs
2.6.2 Riboswitches
2.6.3 Noncoding Small RNAs
2.7 Conclusions
Keywords
References
Part II: Wcb for Chemicals
3. Whole-Cell Catalysts: Sustainable Green-Chemical Producing Entities
4. Microbial Whole-cell Platforms for the Synthesis of Tricarboxylic Acid Cycle-Based Platform Chemicals
5. System-Enabled Microbial Cell Factories for the Production of Biomolecules
6. Biotherapeutic Potential and Properties of Seaweeds
7. Synthesis and Applications of Biopharmaceuticals from Whole-Cell Actinobacterial Isolates
Part III. WCB for Fuels
8. Microbial Consortia: A Mixed Cell Catalyst for Biotransformation of Biomass into Biofuels and Chemicals
9. Biofuels: Fueling the Future with Whole-Cell-Derived Fuels
10. Bioelectrochemical Systems for the Conversion of CO2 into Sustainable Production of Fuels
11. State-of-the-Art of Microbial Whole-Cell Catalysts for Biofuel Production
12. Microbial Fuel Cells: Whole-Cell System for MFC, Current Trends, and Future Prospects for the Green Energy
13. Microalgal Cell: A Machinery for Biofuels Production
14. Microalgal Cell System for Biofuel Production: Present Leads and Future Prospects
15. Recent Advances in Algal Bio-Cathode Powered Microbial Fuel Cells
Part IV: Whole-Cell Biocatalysts for Environment Restoration
16. Production of Biosurfactant by Microbes and Its Application in Biodegradation of Pollutants
17. Biodegradation of Plastic Wastes by Microbial Cells
18. Phototrophic Carbon Capture Using Natural Microalgal Whole-Cell Support: An Eco-Technological Approach
19. Role of Downstream Processing for Production and Purification of Fermentation-Based Products Produced via Whole-Cell Biotransformation
20. Microbial Cell Systems for Polyhydroxyalkanoate Production and Its Diverse Applications
Index

2024-03-14